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Practice Question

When sterilizing your loop in the Bunsen burner, the flame should be colored

Answer Choices:

Correct Answer:

blue.

Rationale:
🔶 Blue — This is correct because the hottest, most efficient part of a Bunsen burner flame is the blue cone, which provides the necessary heat to sterilize the loop until red-hot— 🔶 Yellow — This is incorrect because a yellow flame is a “safety flame” with incomplete combustion, cooler, and sooty, making it unsuitable for sterilization— 🔶 Orange — This is incorrect because an orange flame also indicates incomplete combustion and insufficient heat— 🔶 Red — This is incorrect because the flame itself is never red; instead, the loop glows red-hot after being heated in the blue flame—
Want to practice more questions like this?

This question is from Lab Practical 1 QUIZ WEEK 1-3 -2025 which contains 38 questions.

More Practice Questions
How is an inoculating loop or needle sterilized prior to use?

Answer Choices:

A. exposure to UV radiation for 15 seconds
B. holding in the hottest part of the Bunsen burner flame until red-hot
C. soaking in 95% ethyl alcohol for several minutes and allowing to air dry
D. dipping into a beaker of boiling water
What would happen to peach juice if we apply heat at >135 oc to it such as in the use of an autoclave?

Answer Choices:

A. It would just be filtered
B. It becomes agitated
C. It would become sterile
What is the correct way to open an agar plate to either remove a sample or to inoculate?

Answer Choices:

A. Remove the lid completely and set it on the bench in an upright position.
B. Remove the lid completely, holding it in your hand. not setting it on the bench.
C. Open lid slightly on one side. in a clam-shell fashion.
D. Remove the lid completely and set it on the bench upside down.
From Exam
Lab Practical 1 QUIZ WEEK 1-3 -2025

38 Questions

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Question Details
  • Category: PRE-REQUISITES NURSING COURSES
  • Subcategory: MICROBIOLOGY
  • Domain: MICROBIOLOGY_EXAMS
  • Answer Choices: 4
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