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indirect; direct
⩥ Indirect ELISA detects antibodies in a patient’s serum, which is why it is commonly used for screening HIV, hepatitis, and rickettsial infections.
⩥ In this method, the patient’s antibodies bind to an antigen-coated surface, and then an enzyme-linked secondary antibody is used for detection.
⩥ Direct ELISA, on the other hand, detects antigens from the pathogen itself in patient samples.
⩥ This is particularly useful for diseases like measles or hantavirus, where the virus or antigen is directly identified.
This question is from Microbiology Final which contains 99 questions.
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