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RN Nursing · Medical and Surgical Asepsis · Practice question

Which PPE is first removed by the phlebotomist after drawing blood from a patient in an isolation room?

Answer & explanation

Correct: Gloves

When exiting an isolation room, the correct sequence for removing personal protective equipment (PPE) requires that gloves be removed first. Gloves are considered the most contaminated piece of PPE because they have had direct contact with the patient, the venipuncture site, and potentially blood or body fluids throughout the procedure. By removing gloves first, the phlebotomist prevents the spread of pathogens from the glove surfaces to other PPE or to clean surfaces. After gloves are removed and hand hygiene is performed, the gown is removed next (touching only the inside), followed by eye protection if worn, and then the mask or respirator last. The mask is removed last because it protects the respiratory tract; keeping it in place until the very end minimizes inhalation exposure to any airborne or droplet particles that may still be present near the doorway. Removing the surgical mask or N95 before the gloves would expose the phlebotomist to contaminated glove surfaces while reaching up to the face, which is precisely the type of self-inoculation the PPE removal sequence is designed to prevent. This sequence is established by the CDC and is a core element of standard and transmission-based precaution protocols.

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